Enzymes

ENZYMES

INTRODUCTION:
Enzymes are proteins produced by living cells. They act as biochemical catalysts meaning they lower the activation energy needful for a biochemical reception to occur.
A substrate, is the substance to be acted upon, it binds to the active site of an enzyme to induce the biochemical reaction and fix the desired product. Each active site of the enzyme is unique to the substrate it will bind with.
However, the activeness of an enzyme can be touch by many factors such as pH and temperature and front line of inhibitors because these factors can alter the structure of the enzyme and denature it. When the temperature and pH are higher or lower than what is optimal for the enzyme to work, this when the enzymes performance will be affected; it can either be disabled or slowed down.
However the inhibitor binds to the active site of an enzyme and tug it, which will prevent the substrate to bind to it, and therefore the reaction will slow down.

In this lab we will running the effects of enzymatic activity by conducing 3 look intos, to con how changes in the temperature, pH, and presence of inhibitors will cause this effect.
EXPERIMENT 1
Enzyme activity: the effect of temperature.




Materials:
Potato extract
Catechol
5 analyse vacuum tubes
Test tube holder
Ice bath
Warm bath
Boiling bath
Pen
Ruler
Procedure:
1.With pen and formula mark 1cm and 2cm from bottom on each 5 test tubes.
2. assemble tube 1 till 1cm mark with distilled water.
3.Fill test tubes 2, 3, 4, 5 with potato extract till 1 cm mark.
4.Place tube 2 in stewing water bath.
5.Place tube 3 in speedy water bath.
6.Place tube 4 in sparkler bath.
7.Place tube 5 in test tube rack, on room temperature.
8.Leave the tubes for 5 minutes.
9.Add catechol in all 5 tubes up till the 2cm mark.
10.Observe the simulation change for the next 20 minutes.
Observation and results:
In this experiment we can identify:
The substrate: catechol
The enzyme: catechol oxidase
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